Circulating adult stem and progenitor cell numbers—can results be trusted?

Table 2 Results from flow cytometry analysis

Gated cells as percentage of (grand) parent population	DGC	RBCL
Doublets (%)	1.3 ± 1.1	7.5 ± 2.8**
LYM (%)	82.5 ± 4.4	44.8 ± 4.5***
Live LYM (%)	95.8 ± 0.7	95.3 ± 0.4
Early-apoptotic LYM (%)	4.2 ± 0.7^§§§	4.7 ± 0.4^§§§
Late-apoptotic LYM (%)	0.002 ± 0.000^§§§	0.001 ± 0.000^§
Necrotic LYM (%)	0.006 ± 0.008	0.002 ± 0.002^§
MONO (%)	12.3 ± 2.5	4.6 ± 0.6***
Live MONO (%)	30.4 ± 4.1	62.3 ± 6.1***
Early-apoptotic MONO (%)	69.6 ± 4.1	37.6 ± 6.0***
Late-apoptotic MONO (%)	0.08 ± 0.02	0.05 ± 0.04
Necrotic MONO (%)	0.012 ± 0.007	0.020 ± 0.013
CPC (%LYM)	0.05 ± 0.01	0.06 ± 0.02
CPC (%MNC)	0.05 ± 0.01	0.05 ± 0.02

Data are given as arithmetic mean ± standard deviation. n = 6; DGC density gradient centrifugation, RBCL red blood cell lysis, LYM lymphocytes, MONO monocytes, CPC hematopoietic stem and progenitor cells, MNC mononuclear cells; significant differences between cell isolation techniques and between LYM and MONO within the same quadrant and cell isolation technique are indicated as follows: **p < 0.01, ***p < 0.001 and ^§p < 0.05, ^§§§p < 0.00, respectively

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