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Table 2 Results from flow cytometry analysis

From: Circulating adult stem and progenitor cell numbers—can results be trusted?

Gated cells as percentage of (grand) parent population DGC RBCL
Doublets (%) 1.3 ± 1.1 7.5 ± 2.8**
LYM (%) 82.5 ± 4.4 44.8 ± 4.5***
Live LYM (%) 95.8 ± 0.7 95.3 ± 0.4
Early-apoptotic LYM (%) 4.2 ± 0.7§§§ 4.7 ± 0.4§§§
Late-apoptotic LYM (%) 0.002 ± 0.000§§§ 0.001 ± 0.000§
Necrotic LYM (%) 0.006 ± 0.008 0.002 ± 0.002§
MONO (%) 12.3 ± 2.5 4.6 ± 0.6***
Live MONO (%) 30.4 ± 4.1 62.3 ± 6.1***
Early-apoptotic MONO (%) 69.6 ± 4.1 37.6 ± 6.0***
Late-apoptotic MONO (%) 0.08 ± 0.02 0.05 ± 0.04
Necrotic MONO (%) 0.012 ± 0.007 0.020 ± 0.013
CPC (%LYM) 0.05 ± 0.01 0.06 ± 0.02
CPC (%MNC) 0.05 ± 0.01 0.05 ± 0.02
  1. Data are given as arithmetic mean ± standard deviation. n = 6; DGC density gradient centrifugation, RBCL red blood cell lysis, LYM lymphocytes, MONO monocytes, CPC hematopoietic stem and progenitor cells, MNC mononuclear cells; significant differences between cell isolation techniques and between LYM and MONO within the same quadrant and cell isolation technique are indicated as follows: **p < 0.01, ***p < 0.001 and §p < 0.05, §§§p < 0.00, respectively