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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Generation of insulin-secreting cells from mouse gallbladder stem cells by small molecules in vitro

Fig. 5

Induction of gallbladders stem cell differentiation into insulin-secreting cells in vitro. a Plasmid maps of the Insulin2 Promoter-Cherry and CMV Promoter-Cherry. b, c Phase contrast and red fluorescence view of gallbladder stem cells infected with the lentivirus. Scale bar, 100 μm. d Screening strategy to identify the best medium composition for induction. Pink round shape indicates Cherry+/insulin+ cells. e Representative images of cherry fluorescence in the second round screening. Scale bar, 50 μm. f Secreted insulin levels from each well of the second round screening. *p < 0.05 (compared with each group)

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