Fig. 7

Characterization of gPB cells in vivo. a Schematic strategy of transplanted gPB cells. Cells were first seeded into the sponge and then put into epididymal pad site of diabetic mice. b Phase contrast and fluorescence images of the gPB cells after being seeded into the sponge. Scale bar, 200 μm. c Immunostaining of insulin, Pdx1, and Nkx6.1 after gPB cells are seeded into the sponge. The nuclei were counterstained with DAPI. Scale bar, 100 μm. d Monitoring of fasting blood glucose level of each group. STZ, diabetic mice without transplantation. gPB cells, diabetic mice were transplanted with gPB cells. Gallbladder cells, diabetic mice transplanted with gallbladder stem cells. Normal, normal mice. Green arrow indicates transplantation time. *p < 0.05 comparing the two cell groups (the gPB cells group and gallbladder cells group) on the same day with paired t test. Data presented as mean ± SEM. e Glucose tolerance tests of each group. Blood glucose was measured at 0, 15, 30, 90, and 120 min after glucose stimulation