Fig. 2

Wnt1 is a putative target of miR-326-5p in EPCs. a Binding motif of miR-326-5p on position 243–249 of Wnt1 3′UTR. b Luciferase of the pmirGLO vector containing Wnt1 gene was constructed. c Relative Rluc/Luc ratio (n = 3). *P < 0.01, Wnt1-WT+miR-326-5p vs. Wnt1-WT+NC. d The expression level of miR-326-5p was determined by RT-qPCR in three groups (NC group: EPCs; miR-326-5p antagomir group: EPCs transfected with miR-326-5p antagomir; miR-326-5p agomir group: EPCs transfected with miR-326-5p agomir; n = 3, respectively). e mRNA expression of Wnt1 in the NC group, miR-326-5p antagomir group, and miR-326-5p agomir group (n = 3). f Protein expression of Wnt1 was detected by Western blot in the NC group, miR-326-5p antagomir group, and miR-326-5p agomir group (n = 3). g Qualification of Wnt1 protein expression level (n = 3). The P values for comparison were indicated in the images, respectively