Fig. 3

miR-326-5p promoted EPC angiogenesis in vitro and in vivo. a Tube formation assay on Matrigel was assessed 6 h after seeding HUVECs pretreated with DiI-labeled EPCs/NC, miR-326-5p agomir EPCs, miR-326-5p antagomir EPCs, or miR-326-5p agomir EPCs+ Wnt1 agonist. DiI-positive incorporated EPCs were observed in the HUVEC-formed tube structure (red). Bar, 200 μm. b The number of DiI-positive incorporated EPCs was counted and averaged in each group (n = 4). c Gross look of Matrigel plugs. Bar, 5 mm. d Capillaries of immunofluorescence were observed (red, bar 50 μm) in the sections of Matrigel plugs and e were counted and averaged in each group (n = 5). The P values for comparison were indicated in the images, respectively