Fig. 6

Histological analyses of arteriole density and capillary density in the peri-infarct myocardium. After MI was inducted, miRNA-326-5p-EPCs (EPCs were transfected with miRNA-326-5p), miRNA-326-5p-EPCs+ Wnt1 agonist, EPCs-NC, and PBS (control) were injected into the peri-infarct zones. Heart samples in each group were harvested at 28 days after the operation and then were systemically perfused by BS1 lectin. a Capillaries were observed as tubular structure (red) in the peri-infarcted zone. Bar, 50 μm. b Capillary density was indicated by counting the numbers of the tubular structure and averaged in each group. c Arterioles were visualized by staining heart sections with anti-α-smooth muscle actin(α-SMA) antibody (red) in the peri-infarct zone. Bar, 50 μm. d The numbers of arterioles were counted and averaged in each group. The P values for comparison were indicated in the images, respectively