Fig. 4

Detection of transplanted E-MNCs. a At 2 weeks after IR, GFP-expressing cells were detected in the microvessels and parenchyma (× 200) in the submandibular glands. The blood vessels were stained by ILB4. b The white box area in a was magnified (scale bar, 20 μm) (× 400). At 2 weeks, GFP-expressing cells (white arrow) were recognized clearly in vascular epithelia and parenchyma at the periphery of blood cells in the submandibular glands. Green, transplanted GFP-positive E-MNCs; red, blood vessels stained by ILB4; blue, DAPI. c At 3 weeks, they were detected only in the microvessels (scale bar, 200 μm) (× 200). d, e The white box areas in c were magnified (scale bar, 20 μm) (× 400). Green, transplanted GFP-positive E-MNCs; red, blood vessels stained by ILB4; blue, DAPI. f, g The graphs show the number of observed EGFP-expressing cells (× 200) in microvascular endothelium and perivascular parenchyma of gland tissues in E-MNC-transplanted mice at 2 weeks post-IR (f) and at 3 weeks post-IR (g)