Table 4 Contents in semisolid culture for EPC-CFA
Contents | Company, catalog no. | Final concentration |
|---|---|---|
rm VEGF | Peprotech, #450-32 | 50 ng/mL |
rm basic FGF | Peprotech, #450-33 | 50 ng/mL |
rm EGF | Peprotech, #315-09 | 50 ng/mL |
rm IGF-1 | Peprotech, #250-19 | 50 ng/mL |
rm SCF | Peprotech, #250-03 | 100 ng/mL |
rm IL-3 | Peprotech, #213-13 | 20 ng/mL |
Heparin | YOSHINDO, #121-154 | 2 U/mL |
FBS | SAFC Biosciences, #12303 | 30% |