Fig. 5

Histological changes induced in skin by hADSCs and SVF. a Hematoxylin and eosin (H&E) images of wound edge skin at days 0 and 9 post-injury. Dotted lines outline the boundary between the epidermis and the dermis. b Quantification of the epidermal thickness was performed using Image-Pro Plus 6. (***P < 0.001, compared to control (CON)). c Masson trichrome staining of sections of the wound edges from control and treated mice at 0 and 9 days after wounding. d Quantification of the Masson-positive area was performed using Image-Pro Plus 6. (*P < 0.05, **P < 0.005 compared to CON). e Immunohistological analysis of wound sections from control and treated mice 9 days after injury using antibodies directed against CD31. Arrows mark the CD31+ cells. f Quantification of the CD31-positive cells was performed using Image-Pro Plus 6 analysis software. (*P < 0.05, **P < 0.005 compared to CON). g Quantification of the microvessel density in sections as determined by CD31 expression (*P < 0.05, **P < 0.005 compared to CON)