Fig. 3
From: Direct conversion of human fibroblast to hepatocytes using a single inducible polycistronic vector
Characterization of iHEPDOX. HDF-LTDOX were reprogrammed to iHEP-LTDOX by incubation in HMM media containing 250 ng/mL DOX for 10 days. iHEP-LT are included as reference (see Fig. 1b for details). a mRNA level of multiple hepatic genes was quantified by qRT-PCR and expressed relative to human liver. b Representative fluorescence images of cells immunostained with antibodies against human albumin and a1-antitrypsin. Nuclei were stained with DAPI. Bar equals 40 μm. c PAS staining. d Glutamine uptake from cell media. e Glutamate secretion to cell media. f Human albumin contained in 24-h cell media was quantified by ELISA. PHH: cultured primary human hepatocytes (data extracted from [16]). g Human albumin present in sera from cell transplanted SCID mice quantified by ELISA at 7 and 30 days. M: mouse number