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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Radio-detoxified LPS alters bone marrow-derived extracellular vesicles and endothelial progenitor cells

Fig. 1

Effect of local chest irradiation and RD-LPS treatment. a A schematic illustration of the experimental setup. Hearts of C57BL/6 mice received a 16-Gy single dose of X-ray radiation. b Kaplan-Meyer diagram shows the changes of survival in animals treated with PBS, PBS+16 Gy of X-ray, RD-LPS (20 μg/mouse) or 16 Gy combined with RD-LPS (n = 9–10/group). p < 0.001, Log-rank Mantel-Cox test. c Representative images of histopathologic changes at 250 days after local heart irradiation. Interstitial fibrosis and degeneration of cardiomyocytes after 16 Gy are indicated (arrows). Van Gieson staining, × 20 objective magnification. Scale bar: 50 μm. d Morphometric analysis of cardiomyocyte cross-sectional area 250 days post-treatment. Original magnification × 20. Quantitative analysis of myocyte cross-sectional area is shown; *p < 0.05 and ***p < 0.005 vs. PBS-treated animals. e RD-LPS protects against the radiation-induced accumulation of deleted mitochondrial DNA. Copy number was evaluated with qPCR as detailed in “Materials and methods”. f Flow cytometry analysis of EPCs in the BMCs and g in the circulation. One-way ANOVA, **p < 0.01; ***p < 0.001, n = 6–12/group. Mean ± SD values are shown

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