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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Radio-detoxified LPS alters bone marrow-derived extracellular vesicles and endothelial progenitor cells

Fig. 3

Validation of the expression and function of IFITM3 in BMC-derived sEVs. a Data (mean ± SD) measured using ELISA (n = 5). Unpaired Student’s t test, *p < 0.05. b BMC-derived sEVs from PBS- or RD-LPS-treated mice were stained with anti-CD81 and anti-IFITM3 and were analyzed by flow cytometry. Within Q2 quadrants, numbers indicate the relative percentages of positive cells. Double (CD81 and IFITM3) positive sEVs are shown by arrows. Left: isotype control, middle: PBS-injected group and right: RD-LPS group. c Loss of IFITM3 reduces BM-derived sEV-mediated modulation of function of EPCs. Differentiated EPCs were treated with the conditioned medium either of sh-control-transduced BMCs or sh-control-transduced and RD-LPS-treated BMCs or sh-IFITM3-transduced and RD-LPS-treated BMCs. DiI-ac-LDL uptake was measured by flow cytometry, the measurements were repeated three times, and the panel shows mean ± SD, **p < 0.01

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