Skip to main content
Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Protective mechanism of artemisinin on rat bone marrow-derived mesenchymal stem cells against apoptosis induced by hydrogen peroxide via activation of c-Raf-Erk1/2-p90rsk-CREB pathway

Fig. 1

Rat BMSC culture morphology (a–c), CD marker expression being evaluated by FACS (d), osteogenic and adipogenic differentiation induction (e, f). a The fourth day after isolation. b Passage 3. c Passage 5. d The expression levels of mesenchymal markers CD29, CD90, and hematopoietic marker CD45. e Differentiation potential to osteocytes. Cells were cultured in osteogenic differentiation medium for 21 days followed by the Alizarin Red S (ARS) staining. The representative images were presented here. Control, cultured with normal medium; osteogenic, cultured with osteogenic differentiation medium. f Differentiation potential to adipocytes. Cells were cultured in adipogenic differentiation medium for 21 days followed by the Oil Red O (ORO) staining. The representative images were presented here. Control, cultured with normal medium; adipogenic, cultured with adipogenic differentiation medium

Back to article page

Stem Cell Research & Therapy

ISSN: 1757-6512