Skip to main content
Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Protective mechanism of artemisinin on rat bone marrow-derived mesenchymal stem cells against apoptosis induced by hydrogen peroxide via activation of c-Raf-Erk1/2-p90rsk-CREB pathway

Fig. 6

Artemisinin activation of Erk1/2 but not PI3K is involved in the protective effect. a–c BMSCs were pretreated with various concentrations of artemisinin for 1 h or 1.0 μM artemisinin for up to 160 min. Cell lysates were submitted for western blotting to measure phosphorylation (P) and total level (T) of Erk1/2 and Akt (n = 3). d, e BMSCs were preconditioned with 10 μM PD98059 or LY294002 for 30 min and then treated with 1.0 μM artemisinin for 1 h followed by exposure to 200 μM H2O2 for 24 h, and thereafter, the cell viability was determined using MTT assay (n = 3). a, b *p < 0.05, **p < 0.01, ***p < 0.005 versus control (0 μM in a, 0 min in b); d, e *p < 0.05, **p < 0.01, ***p < 0.005 versus control

Back to article page