Fig. 5

B lymphocytes play a role by affecting T lymphocytes. a Peripheral lymphoid tissues at the peak phase of EAE mice were selected. After positively sorting B cells, the remaining cells, T cells and other immune cell components, were cultured in vitro. Four subtypes of T cells were detected after in vitro treatment of BMSC supernatant. b Statistical analysis of the four subtypes of T cells after in vitro treatment of BMSC supernatant, mean ± SEM, n = 4. c We separately sorted the T cells (CD4⁺) and B cells (CD19⁺) in the peripheral immune tissues of the EAE model mice and co-cultured them in vitro. Changes in the proportion of B cells co-cultured after in vitro treatment of BMSC supernatant, mean ± SEM, n = 4, ***P < 0.001. d Changes in the proportion of T cells co-cultured after in vitro treatment of BMSC supernatant, mean ± SEM, n = 4, *P < 0.05, ***P < 0.001