Fig. 6

Bioinformatics analyses of RNA-seq transcriptional profiling of RPTEC/TERT1 monolayers following 5-day exposure to high glucose (HG) and mannitol (MAN) culture conditions in the presence or absence of MSC-containing transwells: a Table summarising the non-MSC- (control) and MSC-co-cultured (MSC) samples subjected to RNA-seq. b Scatter plot of principal component analysis of the RNA-seq profiles. Each dot represents one sample (nomenclature as per Fig. 5a). c Figures illustrating the numbers of total upregulated and downregulated DEGs for control vs. MSC-co-cultured RPTEC/TERT1 cells under high glucose (HG) and mannitol (MAN) culture conditions. Overlapping segments indicate the numbers of DEGs that were common to both comparisons. d, e Scatter graphs indicating the enrichment factors and number of DEGs of the top 20 enriched pathways for control vs. MSC-co-cultured RPTEC/TERT1 monolayers under HG (d) and MAN (e) conditions based on KEGG pathway enrichment analysis