Fig. 7

Validation of the effect of BM-MSC on RPTEC-TERT1 expression of inflammation-related transcripts. Graphical representations of qRT-PCR analysis of the relative expression of RNA transcripts for IL-6, MCP-1 (CCL2), IL-1β, IL-8, TNFα and NGAL (LCN2) in RPTEC/TERT1 cell monolayers cultured for 5 days in high glucose (HG) (a) or mannitol (MAN) (b) in the presence of BM-MSC-containing transwells (MSC) or no cells (control) for the final 2 days of culture. Results are expressed as mean ± SD for n = 3 samples per condition of the fold difference for MSC vs. control as determined relative to the housekeeping gene RPLP0 using the 2^–∆∆Ct method. Statistical analysis by Student’s unpaired t test, **p < 0.01, *p < 0.05, ns = not significant