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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Exosomes derived from human CD34+ stem cells transfected with miR-26a prevent glucocorticoid-induced osteonecrosis of the femoral head by promoting angiogenesis and osteogenesis

Fig. 3

Angiogenesis was enhanced by miR-26a-CD34+-Exos in vitro. a Uptake of CD34+-Exos and miR-26a- CD34+-Exos shown by fluorescence microscopy. The PKH26 red-labeled Exos were localized in the perinuclear region of HUVECs. Scale bar: 200 μm. b The proliferative effects of different treatments on HUVECs were tested by CCK-8 assay. c Scratching experiment for evaluating the migration capacity of HUVECs at 0, 12, and 24 h in the different groups. Scale bar: 100 μm. d Quantitative analysis of the migration area of HUVECs. e The migration capacity of HUVECs was investigated by transwell assay in different groups. Scale bar: 100 μm. f Quantitative analysis of cell migration by transwell assay. The number of migrated cells was calculated. g Tube formation assay for detecting the tube-forming ability of HUVECs in the different groups. Scale bar: 100 μm. h Quantitative analysis of tube formation. The value of the total mesh area, total length, and number of nodes were measured. *P < 0.05, **P < 0.01

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Stem Cell Research & Therapy

ISSN: 1757-6512