Fig. 1
From: Supplementation of vitamin C promotes early germ cell specification from human embryonic stem cells
Generation of BLIMP1-mkate2 reporter knockin hESC lines. a Schematic illustration of the BLIMP1 locus, and the donor construct carrying T2A-mKate2 and hEF1a-Neo-pA fragments. Black boxes indicate the exons. b Screening by PCR of the homologous recombinants for BLIMP1-mkate2 and of the removal of the selection cassettes (loxP-hEF1a-Neo-pA-loxP). The clones bearing BLIMP1-mkate2 were selected for use in the subsequent studies. c A phase-contrast image of the BLIMP1-mkate2 reporter knockin hESCs. Scale bar = 200 μm. d FACS analysis for OCT3/4, SOX2, SSEA4, TRA-1-60, and NANOG expression in reporter knockin hESCs