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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Supplementation of vitamin C promotes early germ cell specification from human embryonic stem cells

Fig. 2

Induction of PGCLCs from BLIMP1-mkate2 reporter knockin hESCs with a two-step method. a Schematic protocol for PGCLCs induction. b, c FACS analysis of mKate (+) cell induction by aRB27 medium and GK15 medium at day 4 with different pre-induction time; n = 3 independent experiments. d FACS analysis of mKate (+) cell induction at day 4 stimulated by different concentrations of LIF (1 μg/ml or 10 ng/ml) and BMP4 (0, 200, 500 ng/ml); n = 3 independent experiments. e Bright field (left) and fluorescence images (right) of day 4 embryoid bodies (EBs) stimulated by cytokines; scale bar = 200 μm; n = 3 independent experiments. f FACS analysis of mKate and TNAP double-positive cells at day 4; n = 3 independent experiments. g FACS analysis of EpCAM and INTEGRINα6 double-positive cells at day 4; n = 3 independent experiments. h Expression analysis by RT-qPCR for day 4 EBs differentiated form BLIMP1-mkate2 reporter knockin hESCs. Relative expression levels are shown with normalization to hESCs or day 4 EB. n = 3 independent experiments. Data are presented as mean ± SD

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