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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: NT3P75-2 gene-modified bone mesenchymal stem cells improve neurological function recovery in mouse TBI model

Fig. 1

NT3P75-2 induction improves the cell growth rate of NSC-34 and PC12 cells in vitro. a NT3 expression levels of GFP, NT3, and NT3P75-2 in NSC-34 were analyzed by western blot, and the statistical data was also presented (#P < 0.05, *P < 0.05, ***P < 0.001 by one-way ANOVA followed by Bonferroni’s multiple comparison test, n = 3). b NT3 expression levels of GFP, NT3, and NT3P75-2 in PC12 were analyzed by western blot, and the statistical data was also presented (#P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA followed by Bonferroni’s multiple comparison test, n = 3). c NT3 expression levels of GFP, NT3, and NT3P75-2 in HEK293T were analyzed by western blot, and the statistical data was also presented (**P < 0.01 by one-way ANOVA followed by Bonferroni’s multiple comparison test; n.s., no significance, n = 3). d NT3 concentrations of cellular supernatant of GFP, NT3, and NT3P75-2-infected NSC-34 cells were assessed by ELISA (***P < 0.001 by one-way ANOVA followed by Bonferroni’s multiple comparison test; n.s., no significance, n = 6). e NT3 concentrations of cellular supernatant of GFP, NT3, and NT3P75-2-infected PC12 cells were assessed by ELISA (***P < 0.001 by one-way ANOVA followed by Bonferroni’s multiple comparison test; n.s., no significance, n = 6). f NT3 concentrations of cellular supernatant of GFP, NT3, and NT3P75-2-infected HEK293T cells were assessed by ELISA (#P < 0.05, ***P < 0.001 by one-way ANOVA followed by Bonferroni’s multiple comparison test, n = 6). g CCK8 assay measurement of cell growth of NSC-34 cells infected with lentiviruses of GFP, GFP-NT3, or GFP-NT3P75-2 (#P < 0.05, ##P < 0.01, ###P < 0.001, **P < 0.01, ***P < 0.001 by two-way ANOVA followed by Bonferroni’s multiple comparison test, n = 6). h CCK8 assay measurement of cell growth of PC12 cells infected with lentiviruses of GFP, GFP-NT3, or GFP-NT3P75-2 (##P < 0.01, ###P < 0.001, **P < 0.01, ***P < 0.001 by two-way ANOVA followed by Bonferroni’s multiple comparison test, n = 6). i CCK8 assay measurement of cell growth of NSC-34 cells after treatment with the supernatant of the HEK293T cells which were respectively infected with lentiviruses of GFP, NT3, or NT3P75-2 (##P < 0.01, *P < 0.05, ***P < 0.001 by two-way ANOVA followed by Bonferroni’s multiple comparison test, n = 6). j CCK8 assay measurement of cell growth of PC12 cells after treatment with the supernatant of the HEK293T cells which were respectively infected with lentiviruses of GFP, NT3, or NT3P75-2 (###P < 0.001, **P < 0.01, ***P < 0.001 by two-way ANOVA followed by Bonferroni’s multiple comparison test, n = 6)

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