Fig. 1

Morphology of lipoma-derived cells. a, b Histological 5-μm paraffin section of lipoma (LP) stained with hematoxylin and eosin (H&E). a The central part (ct) of LP shows the typical morphology of the adipose tissue while the peripheral capsule was formed of dense connective tissue layer. b Lipoma capsule (pf) shows longitudinally oriented collagen fibers with numerous capillaries (black arrow). c–e Phase contrast (PC) images of ASCs harvested from retroperitoneal (c, RP), subcutaneous (d, SC), and lipoma (e, LP) adipose tissue exhibit the typical spindle-shaped fibroblast-like morphology of stem cells under culture condition. (f–w) Immunofluorescence images show CD44 (green, i–k), CD90 (red, l–n), and CD73 (green, o–q) positive ASCs of RP, SC, and LP fat, respectively. r–w Immunofluorescence images show CD45 (r–t) and CD34 (u–w) negative ASCs of RP, SC, and LP fat, respectively. Cells from all experimental groups with no added primary antibodies were processed in parallel and were served as negative controls (NC, f–h). Scale bar in a, b = 0.5 mm, c–e = 100 μm, and f–w = 20 μm