Fig. 2
Evaluation of the cell viability and cell proliferation. a FACS analysis of ASCs harvested from retroperitoneal (RP), subcutaneous (SC), and lipoma (LP) adipose tissue. A volume of 100 μL of cell suspension (2 × 106 per mL) per well was transferred into a 96-well-culture plate. The plate was centrifuged and the supernatant was discarded. The pellets were preblocked in 1% BSA in PBS supplemented with 0.01% NaN3 and 0.5% goat serum and 10% horse serum. The pellets were incubated with 50 μL of CD44, CD90, CD105, CD45, and MHCII primary antibodies. FACS analysis shows the percentage of CD44, CD90, and CD105 immunopositive ASCs. The selected cell populations show weak immunoreaction against CD45 and MHCII. b Evaluation of cell viability using MTT assay, the absorbance was measured at 570 nm. The data analysis revealed significant increases cell viability of RP fat-derived ASCs compared to both SC and LP derived cells. c Quantification of total protein contents of RP, SC, and LP derived ASCs cultivated under growth condition for 48 h. Sulforhodamine B assay (SRB) measures the protein contents indicative for cell number. The analysis shows higher protein contents of the RP derived cells compared to SC and LP derived cells. The SC derived cells exhibit more cells compared to LP derived cells. All data presented as mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0. 001