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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Investigation of stemness and multipotency of equine adipose-derived mesenchymal stem cells (ASCs) from different fat sources in comparison with lipoma

Fig. 4

Adipogenic differentiation of ASCs. ASCs harvested from retroperitoneal (RP), subcutaneous (SC), and lipoma (LP) fat sources were cultivated in the presence of basal medium (BM). ad Photomicrographs of RP, SC, and LP derived ASCs stained with ORO staining show fat vacuole formation (red) following adipogenic differentiation induction. The cells of LP exhibit an impaired adipogenic differentiation capacity. e Semi-quantification of the ORO staining RP, SC, and LP derived ASCs was performed at 492-nm absorbance. RP fat-induced cells show a higher ORO values compared to both SC and LP induced cells as well as those cells cultivated in BM. fh Expression fold change of FABP4 (f), PPARγ (g), and LEP (h) at day 10 post adipogenic differentiation induction show upregulation of adipogenic markers in RP and SC derived cells compared to LP cells. The expression was normalized to non-induced cells in BM using 2−∆∆cq method [30]. The data presented as mean ± SEM. *p < 0.05, **p < 0.01, and ***p < 0.001. Scale bar = 100 μm

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