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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Extracellular vesicles derived from human Wharton’s jelly mesenchymal stem cells protect hippocampal neurons from oxidative stress and synapse damage induced by amyloid-β oligomers

Fig. 3

Uptake of hMSC-EVs is primarily carried out by non-neuronal cells in hippocampal cultures. a Vybrant DiI-labeled hMSC-EVs were incubated for 24 h with hippocampal cultures following previous incubation of cultures for 24 h with 500 nM AβOs. White arrows indicate representative uptake of hMSC-EVs by MAP 2/GluA1-positive cells. b Analysis of co-localization of Vibrant DiI and MAP 2/GluA1 labeling revealed that uptake of hMSC-EVs was mostly carried out by non-neuronal cells in culture. Quantification was performed on ten images from each of three coverslips in each three independent experiments. c GLT-1-positive astrocytes in hippocampal cultures exhibit robust hMSC-EV uptake. d Representative z-stack maximal intensity projection confocal image demonstrating the presence of Vybrant DiI-labeled hMSC-EVs (red) inside GLT-1-labeled astrocytes (green). Scale bar, 20 μm

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Stem Cell Research & Therapy

ISSN: 1757-6512