Fig. 4
hMSC-EVs rescue AβO-induced ROS formation in hippocampal cultures. a, b Representative DCF fluorescence images from hippocampal cultures exposed to vehicle or 500 nM AβOs for a total period of 6 h. c, d Hippocampal cultures were treated with two different doses of hMSC-EVs (6.1 × 107 particles, identified as [1x], or 1.82 × 108 particles, identified as [3x]). Scale bar, 50 μm. e Integrated DCF fluorescence. Bars represent means ± SEM from three experiments using independent hippocampal culture. In each experiment, three images from each of three coverslips were acquired and analyzed using NIH ImageJ software. *p < 0.05; **p < 0.0001; one-way ANOVA followed by Tukey’s post hoc test