Fig. 5
Inactivation of catalase abolishes the capacity of hMSC-EVs to block oxidative stress induced by AβOs in hippocampal cultures. a Catalase activity of hMSC-EVs was determined by high-resolution respirometry by measuring O2 release in response to increasing concentrations of added hydrogen peroxide. Treatment with aminotriazole (AMZ, a catalase inhibitor) fully inactivated catalase activity of hMSC-EVs. b Integrated DCF fluorescence from hippocampal cultures exposed to AbOs (or vehicle) in the presence of hMSC-EVs previously treated (or not) with aminotriazole. Bars represent means ± SEM from three experiments using independent neuronal cultures. In each experiment, three images from each of three coverslips were acquired and analyzed using NIH ImageJ software. Scale bar, 50 μm. *p < 0.05; **p < 0.0001; one-way ANOVA followed by Tukey’s post hoc test. c–f Representative DCF fluorescence images of hippocampal cultures