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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: The TRIM protein Mitsugumin 53 enhances survival and therapeutic efficacy of stem cells in murine traumatic brain injury

Fig. 1

Growth morphology and immuno-phenotypic characterization of hUC-MSCs. a Wharton’s jelly tissue pieces (red arrow) were plated and cultured for 2 weeks, in order to grow out primary hUC-MSCs (P0) from the tissues. b After 2 days in culture, passage-3 (P3) hUC-MSCs displayed a spindle-shaped or fibroblast-like morphology. c After 4 days in culture, morphology of P3 hUC-MSCs changed to a homogeneous fibroblastoid cell type with a smooth border. Scale bar = 100 μm. d Flow cytometric analysis were used to characterize the P3 hUC-MSCs. hUC-MSCs were positive for CD73, CD90, and CD105 and negative for hematopoietic lineage markers CD34, CD45, and HLA-DR

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