Fig. 2

Depletion of TSG-6 from ASC-CCM fails to suppress microglial activation. a Biochemical assessment of NO release from BV2 cells using the Griess assay. b Assessment of gene expression by Sybr Green real-time PCR and expressed as fold change normalized to internal control (GAPDH) in the study groups. Data represent mean ± SEM from three separate experiments performed in duplicate. *p < 0.05; ***p < 0.001; ^#p > 0.05. c Microglial activity as shown by the increased Iba1 immunoreactivity with LPS and IFNγ-stimulated BV2 cells after 12 h exposure. The bar graph shows the quantification of mean fluorescence intensity of Iba1. Data are mean ± SEM performed in duplicates. ^#p > 0.05; ***p < 0.001. Scale bar = 20 μm