Fig. 1

The purity of sorted GFP+ cells. a Immunostaining in the HC layer showed no GFP+ cells co-labeled with HCs. b In the SC layer, GFP+ cells co-labeled with Sox2+ SCs. c Different ages of Sox2^GFP/+ mouse cochleae were dissected and dissociated into single cells, and the Sox2+ SCs were sorted via flow cytometry. The proportions of Sox2+ cells were 6.19% at P3, 4.59% at P7, 2.07% at P14, and 1.11% at P30. d, e Immunostaining of flow-sorted Sox2+ SCs of different ages showed a high percentage of Sox2+ and GFP+ cells, and no Myo7a+ cells were found. Scale bars are 20 μm in a and b and 50 μm in d and e