Fig. 2
From: Age-related transcriptome changes in Sox2+ supporting cells in the mouse cochlea
The neonatal SCs have greater sphere-forming ability than the older SCs. a The Sox2GFP/+ mice were harvested at P3, P7, P14, and P30. Flow cytometry was used to isolate the different ages of Sox2+ SCs, and these cells were cultured for 5 days. b, c P3 Sox2+ SCs generated significantly more and larger spheres than P7 and P14 Sox2+ SCs, while the P30 Sox2+ SCs could not form spheres. d The cultured cells in the first generation were used for the differentiation assay. e The spheres formed by P3 Sox2+ SCs stained with the HC marker Myo7a. f The spheres formed by P7 Sox2+ SCs stained with the HC marker Myo7a. g The spheres formed by P30 Sox2+ SCs stained with the HC marker Myo7a. h The average number of HCs generated by spheres of each age Sox2+ SCs. i The total number of HCs generated by P3, P7, P14, and P30 Sox2+ SCs. ***p < 0.001. Scale bars are 50 μm in b and 10 μm in e–g