Fig. 3
From: Age-related transcriptome changes in Sox2+ supporting cells in the mouse cochlea
P3 Sox2+ SCs generated more HCs compared with the other three ages of SCs in vitro. a We used the FITC channel to sort P3, P7, P14, and P30 Sox2+ SCs, and we cultured the sorted GFP+ cells at 50 cells/μl for 10 days. b P3 Sox2+ SCs generated a large number of Myo7a+ cells. c P7 Sox2+ SCs also could form colonies and generate Myo7a+ cells. d, e Both P14 and P30 Sox2+ SCs could not form colonies, but the single cells could generate Myo7a+ cells. f P3 Sox2+ SCs formed more Myo7a+ cells compared with P7, P14, and P30 Sox2+ SCs. g Both inside and outside of the colony, P3 SCs formed more Myo7a+ cells compared with P7, P14, and P30 Sox2+ SCs. ***p < 0.001. Scale bars are 10 μm in b–h