Fig. 5

O-GlcNAcylation promoted Sox9 activity and repressed Runx2 activity. CESCs assigned to the Thiamet-G group, in which O-GlcNAcylation of proteins should be relatively high. CESCs assigned to the DON group in which O-GlcNAcylation of proteins should be relatively low. CESCs were induced under low-glucose condition in basic medium for 24 h. a COL2, ALP, and OCN gene expressions were assessed by Q-PCR. b, c COL2, ALP, and OCN protein expressions were assessed and analyzed by western blot. The protein contents were normalized according to GAPDH level. Data represent the mean ± SD (n = 3 independent experiments, one-way ANOVA). *p < 0.05, **p < 0.01, and ***p < 0.001