Table 2 Primer sequences used in this study
Primer for RT-PCR | |
|---|---|
Gene symbol | Primer sequences (5′ to 3′) |
GAPDH-Fa | CTCTCTGCTCCTCCTGTTCG |
GAPDH-Rb | TTAAAAGCAGCCCTGGTGAC |
Sox9-F | TTTGCTTGTTCACTGCAGTCTTAAG |
Sox9-R | GGCATCTGCCTTCCACATG |
COL2-F | GCTCCCAGAACATCACCTACCA |
COL2-R | AACAGTCTTGCCCCACTTACCG |
AGN-F | CACGATGCCTTTCACCACGAC |
AGN-R | TGCGGGTCAACAGTGCCTATC |
Runx2-F | TACAGTAGATGGACCTCGGGAAC |
Runx2-R | GCGGGACACCTACTCTCATACTG |
COL1-F | CACAGAGGTTTCAGTGGTTTGG |
COL1-R | ACCATCATTTCCACGAGCA |
ALP-F | CAAAGGCTTCTTCTTGCTGG |
ALP-R | GGTCAGAGTGTCTTCCGAGG |
OCN-F | CGCCTGGGTCTCTTCACTAC |
OCN-R | CTCACACTCCTCGCCCTATT |