Fig. 4

miR-664-5p targets p53 during apoptosis of granulosa cells. a qRT-PCR was used to detect the expression of miR-664-5p in exosomes of different cells^**P<0.01vs.3T3-exosome group. b After transfection of miR-664-5p mimic and wild-type or mutant vectors into mouse ovarian granulosa cells, dual-luciferase reporter genes were performed to determine luciferase activity^**P<0.01 vs. WT group. c Cell viability and the expression of p53 protein were measured after overexpression of miR-664-5p in mouse ovarian granulosa cells^**P<0.01 vs.control group, ^##P<0.01 vs. cisplatin group. Data are expressed as mean ± standard deviation (SD). Each experiment was repeated three times