Fig. 1

EZH2 is highly expressed in glioma tissues and cells. a The intersection of the top 700 DEGs in GSE12657, GSE35493, and GSE50161. b, c Heat maps of DEGs in GSE35493 and GSE50161. d EZH2 gene expression profile analysis in GSE12657; green represents the gene expression of normal samples, and red represents the gene expression of glioma samples. e EZH2 gene expression determined in normal and glioma samples based on the TCGA database. f Gene network map of EZH2 and its related genes generated using Cytospace software. g EZH2 mRNA level measured in normal brain tissues (n = 12) and glioma tissues (n = 54) using RT-qPCR. h EZH2 protein level measured in normal brain tissues (n = 12) and glioma tissues (n = 54) using Western blot analysis. i Positive expression of EZH2 detected in glioma using immunohistochemistry (200×). j EZH2 expression measured in human normal brain glial cell line HEB and human glioma cell lines (U87, U251, LN229, and A172) using RT-qPCR; *p < 0.05 vs. normal brain tissues/HEB cell line; measurement data were shown as mean ± standard error; comparisons between two groups were conducted by unpaired t test, and comparisons among multiple groups were assessed by one-way analysis of variance (Dunnett’s post hoc test). The experiment was repeated three times to obtain the mean value