Fig. 6

Effect of Akt inhibition on LPS-induced macrophages polarization in response to MSCs treatment. RAW264.7 cells were treated with or without GSK2141795 (30 μmol/l), followed by stimulation with LPS (500 ng/ml) or MSCs for 48 h. a The effect of GSK2141795 on the MSCs/LPS-induced RAW264.7 and peritoneal macrophages phenotype was tested by flow cytometry. b The effect of GSK2141795 on the MSCs/LPS-induced RAW264.7 and peritoneal macrophage phenotype was tested by WB. c Evaluation of the effect of MSCs on the Akt/FoxO1 signaling pathway with WB. d The effect of MSCs on FoxO1 translocated from the nucleus to the cytoplasm were tested by WB. The results are presented as the mean ± SD (n = 3). ###p < 0.001 vs. LPS group; &p < 0.05 vs. MSCs+LPS group; &&p < 0.01 vs. MSCs+LPS group; &&&p < 0.001 vs. MSCs+LPS group. GSK2141795, Akt inhibitor; LPS, lipopolysaccharide; MSCs, mesenchymal stem cells; PMA, peritoneal macrophages; rTGF-β, recombinant TGF-β; WB, Western blot