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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: In vivo conversion of rat astrocytes into neuronal cells through neural stem cells in injured spinal cord with a single zinc-finger transcription factor

Fig. 3

The assessment of apoptosis in adult astrocyte-derived induced neural stem cells (iNSCs). a A growth curve of induced neural stem cells (iNSCs) during different passages. b SA-β-Gal activity in in iNSCs after 3 and 10 passages. c Immunostaining of iNSCs for Caspase 3 and Annexin V after 10 passages. d Quantification of the percentage of Caspase 3-positive and Annexin V-cells after 3 and 10 passages revealed by immunostaining in c. Data in b and d are given as mean ± SD of three independent experiments. Data were analyzed by the unpaired t test. The number of counted cells is presented in Additional file 4: Table S3

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Stem Cell Research & Therapy

ISSN: 1757-6512