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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Osteogenic commitment of Wharton’s jelly mesenchymal stromal cells: mechanisms and implications for bioprocess development and clinical application

Fig. 5

Effect of BM-MSC-derived products on osteogenic commitment of WJ-MSC. a Representative images of AR staining in passage 4 WJ-MSC after 3 weeks of culture with ODM alone (top) or supplemented with either EV or protein fractions collected from undifferentiated BM-MSC (week 0) or BM-MSC cultures at week 1, 2 or 3 of osteogenic differentiation (bottom). b Representative images of AR staining in passage 4 WJ-MSC after 1, 2 or 3 weeks of culture with CM obtained from differentiating BM-MSC cultures at week 1. CM conditioned media, EV extracellular vesicles, ODM osteogenic differentiation media, SN supernatant. In all cases, scale bars: 100 μm

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Stem Cell Research & Therapy

ISSN: 1757-6512