Fig. 5

The process of Dermo1⁺ stem cells in Club cell regeneration in NAPH injury repair. A Establishment of NAPH injury model. Club cells were ablated gradually at day 1 and almost restored at day 7. NE cells increased and served as stem cell during Club cell regeneration. B Dermo1⁺ cells (arrow) appeared in the airway epithelium after NAPH injury displayed as GFP^positive/PGP9.5^positive cells (upper panel) and GFP^positive/CC10^positive cells (lower panel) respectively. C Amplified display of GFP⁺ NE cells (arrowhead) or non-NE cells (arrow) in the airway epithelium at day 1. D Both GFP^positive/CC10^positive cells (arrowhead) and non-Club cells (arrow) in the airway epithelium were detected at day 1. E Dynamic changes of the proportion of CC10^positive/PGP9.5^positive cells in the airway epithelium after NAPH injury. F, G Statistics of GFP^positive/PGP9.5^positive (E) or GFP^positive/CC10^positive (F) cells over total GFP^positive Dermo1 stem cells after NAPH injury. d, days post NAPH injury. Scale bar = 50 μm