Fig. 1
From: Flow-enhanced priming of hESCs through H2B acetylation and chromatin decondensation
Shear flow induces histone H2B acetylation of hESCs. The schematic of iTRAQ labeling (a) was illustrated, where two samples (C1, C2) were used as static control in both LC-MS/MS Run 1 and Run 2. Simple western immunoblots (b) showed H2B and AcH2B proteins under static and shear conditions. Expression abundance of H2B (c) and AcH2B (d) as well as the ratio of AcH2B/H2B (e) were validated with immunoblots. LMNB1 served as loading control in b–e. Data are presented as the mean ± SE of normalized chemiluminescence in three replicates. Here Shear in b–e denotes the steady shear flow of 1.1 Pa for 24 h