Fig. 2

Effects of the ASC-CM on proliferation of HUVECs and NIH-3T3 cells via CCK-8 assay. a Optimization of the ratio of ASC-CM to medium using HUVECs (optimal ratio selected was 4:6). b, c Effects of ASC-CM in medium (4:6) on proliferation of HUVECs and NIH-3T3. d, e Effects of ASC-CM on the coated-surface (4:6) on proliferation of HUVECs and NIH-3T3 at 48 h; HUVECs, human umbilical vascular endothelial cells; FP-CM, facial periosteal cell-conditioned medium; MSC-CM, mesenchymal stem cell-conditioned medium; ASC-CM, antler stem cell-conditioned medium; ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 when compared to 0:10 in a, and compared to DMEM in b–e; ^##p < 0.01 when compared to IGF1; n = 3; mean ± SD