Fig. 6

Effects of ASC-CM on rat wound healing quality. a H&E staining and IHC evaluation of the wound healing tissues. b Number of cutaneous appendages (hair follicles and sebaceous glands)/field (× 40) in the healing tissue. c Number of PCNA-positive cells/field (× 100) in the healing tissue. d Number of vessels/field (× 100) in the healing tissue. Note that ASC-CM group had the highest number of cutaneous appendages and vessels but the least PCNA-positive cells (on day 16 when wound healing reaching completion) compared to other three types of control groups; green arrow, hair follicle; yellow arrow, sebaceous gland; black arrow, PCNA-positive cells; blue arrow, α-SMA-positive vessels; PCNA, proliferating cell nuclear antigen; α-SMA, α-smooth muscle actin; ^**p < 0.01, ^***p < 0.001 compared to DMEM, ^##p < 0.01, ^###p < 0.001 compared to EGF; bar = 1 mm; n = 8/group; mean ± SD