Fig. 8

Fractionation of the ASC-CM components using AKTA protein chromatography. a Cytochrome C served as an internal reference. b DMEM, four peaks identified. c MSC-CM, four peaks identified. d ASC-CM, six peaks identified. e The relative area of peaks in DMEM, MSC-CM, and ASC-CM respectively. Note that the ASC-CM sample had two more peaks appeared at retention volumes of 27 ml and 38 ml than the other two types of media (DMEM and MSC-CM); MSC-CM, mesenchymal stem cell-conditioned medium; ASC-CM, antler stem cell-conditioned medium; each sample in triplicates; f EGF concentration in MSC-CM and ASC-CM. Note that EGF in ASC-CM was highly significantly higher than that in MSC-CM (48.73 ± 7.5 and 86.25 ± 12.4 pg/10⁶ cells, respectively, p < 0.01). ^**p < 0.01, ^***p < 0.001; n = 3; mean ± SD