Fig. 2

S-ADSCs show higher potential in adipogenic differentiation than V-ADSCs through promoting mitotic clonal expansion. S-ADSCs and V-ADSCs prepared as specified in Fig. 1 were subjected to adipogenic induction. a, b After 18 days of adipogenic induction, lipid contents were visualized using Oil Red O staining (a) and quantified by elution of Oil Red O (b). Scale bar 50 μm. c Gene expression of white adipocyte markers was measured by qPCR at days 0, 4, 8, and 12 after adipogenic induction. d, e Mitotic clonal expansion was determined by EdU incorporation assay after 16 h of adipogenic induction. Fluorescence signals were examined by fluorescence microscope (d). Scale bar 100 μm. Growth indices as specified in Fig. 1 are shown (e). Data are presented as mean ± SEM. n = 4–6 per group. **P < 0.01, ***P < 0.001