Fig. 5

CD90 silencing inhibits mitotic clonal expansion of S-ADSCs to influence adipocyte differentiation. S-ADSCs from mice (n = 10) were infected with shCD90 or shControl lentivirus for 72 h and then were subjected to adipogenic induction. a The mRNA level of CD90 was detected by qPCR before adipogenic induction. b, c Mitotic clonal expansion was determined by EdU incorporation assay after 16 h of adipogenic induction. Fluorescence signals were detected by fluorescence microscope (b). Scale bar 100 μm. Growth indices as specified in Fig. 1 are shown (c). d, e After 18 days of adipogenic induction, lipid contents were visualized using Oil Red O staining (d) and quantified by eluting Oil Red O (e). Scale bar 50 μm. f The mRNA levels of white adipocyte markers were measured by qPCR at days 0, 4, 8, and 12 during the induction. Data are presented as mean ± SEM. n = 4–6 per group. *P < 0.05, **P < 0.01, ***P < 0.001