Fig. 8From: Surgical vacuum filter-derived stromal cells are superior in proliferation to human bone marrow aspirateGene expression of the osteoblastic markers. The target genes were normalized to the reference gene GAPDH using the der ΔCt method with ΔCt = Ct test gene − Ct reference gene (GAPDH) and ΔΔCt = ΔCt sample − ΔCt calibrator (unstimulated cells). The relative quantification (RQ) (=fold change compared to the calibrator) was calculated as 2-ΔΔCt. The figure shows the single values of n = 10 patients as symbols with the median as line. BM bone marrow, CB cancellous bone, VF vacuum filter, SF cell saver filtrate reservoir. a RQ of RUNX. b RQ of ALP. c RQ of BMP-2Back to article page