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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Fibroblast growth factor-2 stimulates proliferation of human adipose-derived stem cells via Src activation

Fig. 4

Src activation in FGF-2-treated hASCs. After incubation in serum-free DMEM for 18 h, cells were treated with FGF-2 with/without NVP-BGJ398 (0.1 μM) or PP1 (5 μM). Cell proliferation was assessed with a Cell Counting Kit-8 after culturing for 48 h. The cell lysates were prepared after treatment for 5 min. NVP-BGJ398 or PP1 was added 1 h prior to stimulation with FGF-2 (5 ng/ml). a Immunoblots of p-Src (Tyr416) and p-MEK1/2 under the treatment of cells with FGF-2 with/without NVP-BGJ398. b Effect of PP1 (Src selective inhibitor) (0/0.5/1/2/5 μM) on FGF-2-dependent proliferation of hASCs (n = 6). *p < 0.01 compared with no inhibitor. c Immunoblots of p-Src (Tyr416) and p-MEK1/2 in cells treated with FGF-2 with/without PP1

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