Fig. 4

Purification, single-cell colony, and long-term culture for HB expansion. a Flow cytometric sorting of EpCAM⁺/C-kit⁻ HBs at day 10. b Flow cytometric analyses of EpCAM and Ki67 expression in expanded HBs (passage 10). c Immunostaining analyses of HBs after 10 passage expansion. d Hierarchical clustering analyses of mRNA profiling of expanded HBs (HB-P10 and HB-P20), unexpanded HBs (HB-P0), and iPSCs. e Heat map comparison of the gene expression profiles endodermal, HB, and hepatocyte among expanded and unexpanded different cell clone lines. Representative genes were indicated. Normalized fluorescent intensity values range from red (high) to blue (low) coloring