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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Synergistic modulation of signaling pathways to expand and maintain the bipotency of human hepatoblasts

Fig. 5

Differentiation of expanded HBs into functional hepatocytes in vitro. a Phase-contrast images of differentiating cells. Scale bar 100 μm. b Expanded HB-derived HLCs (HB-P10-Hepatocyte) expressed mature hepatocyte-specific markers. c ALB secretion was analyzed. Data are presented as mean ± SEM, n = 3. d CYP450 activity assay of different origin of hepatocytes. Data are presented as mean ± SEM, n = 3. e Urea secretion among different origin of hepatocytes. Data are presented as mean ± SEM, n = 3. f Periodic acid-Schiff (PAS) staining on different origin of hepatocytes. Scale bar represents 100 μm

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